Background image from Nature Medicine 2004 June: Oligodendrocyte precursor cells in primary culture, which were isolated from the spinal cord 3 days after contusion injury. Improving the ability of such endogenous cells to promote recovery by increasing cAMP levels (see paper by Pearse et al., page 610 may be a new therapeutic target for the treatment of spinal cord injury. Image courtesy of Soonmoon Yoo and Jean Wrathall.
Cold Spring Harbor Laboratory
Regulatory & Non-coding RNAs
05.15.2018 - 05.19-2018
Molecular Biology in Single Cells Symposium
MY LATEST RESEARCH
Endogenous mRNAs localize into distal axons of sensory neurons. Confocal FISH-IF images of naïve vs. injured L4-5 DRGs and sciatic nerves in vivo (left panel) and DRG cultures (right panel) show increased signal for NMP35 (left panel, arrows) and amphoterin (right panel, arrowheads) mRNAs in distal axons whereas a decrease in the neuronal cell bodies. Scale bars, 3 μm for nerves (axons) and 10 μm for DRGs.
The heatmap with a cluster dendrogram shows distinct patterns of miRNA levels in sciatic nerve in response to injury. Red shows an increased change compared to the uninjured control and green denotes a decreased level.
Local processing of axonal pre-miRNA in isolated axons. Top. Schematic of an in vitro compartment cell culture system for isolation of axons. Bottom. Relative levels of pre- and mature miRNA-148b in the isolated axons. Data (mean ± SD, n=3) are normalized to the levels at 0 h.